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The functionality was confirmed by analyzing the stabilization of the mechanical hysteretic response to strain levels up to 8%.
A cytosolically located, cofactor-balanced isobutanol pathway, consisting of a mosaic of bacterial enzymes whose in vivo functionality was confirmed by complementation of null mutations in branched-chain amino acid metabolism, was expressed in S. cerevisiae.
Glycosylation was analyzed by mass spectroscopy; functionality was confirmed by antigen ELISA, as well as rabies and pseudotype virus neutralization.
The diversity of each library was estimated to be between 1 and 2.0 × 10 different clones, and their functionality was confirmed by biopanning on human serum albumin (HSA).
GGCX-nanodisc catalytic functionality was confirmed by monitoring carboxylation of the pentapeptide substrate FLEEL after protein incubation at 25°C for up to 24 h.
Their functionality was confirmed by searching the P. trichocarpa ESTs database http://www.populus.db.umu.se/index.html[ 63] in order to have an additional support in the discovery of the most promising CGs.
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NH2-MCM-41 was prepared using a post-grafting process and the successful amino-functionality was confirmed by Fourier transform infrared (FTIR) and X-ray energy dispersive (EDS) spectra.
Transcriptional functionality was confirmed as an evolutionary basis for this conservation by coincidence of recurrent blocks with cis-elements and protein-binding sites.
Both assays revealed significant and comparable loss of cell functionality that was confirmed by DNA assay.
The IR spectrum of 1 exhibited absorption bands for hydroxyl (3440 cm−1) and α,β-unsaturated ketone (1701 and 1658 cm−1) functionalities, which was confirmed by the UV absorption at λ max (MeOH) 237 nm.
The functionality of R77H was confirmed by replicating findings in COS7 cells expressing variant-specific CD11b.
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