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Figure 10B shows functionality of the domain in a cell-based assay and a model system for biofilm formation.
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Recent studies have suggested these names do not accurately reflect the structures or functionality of the domains.
Figure 3B shows that mutation of the Grb14 FLVRS motif abolishes tyrosine phosphorylation of Grb14 suggesting the functionality of this domain must be intact for Tie2 mediated Grb14 tyrosine phosphorylation.
DHCR24 contains a highly conserved FAD (flavin adenine dinucleotide -binding dinucleotide -bindingedomainn of desmosterol to cholesterol is dependent on FAD [ 7], suggesting functionality of the conserved domain.
With the lack of high sequence conservation of the C-terminal end with other species, it is difficult to determine the importance or functionality of this domain.
This suggested that careful deletion of the linker might improve crystallization without eliminating the inhibitory functionality of the Ubl domain.
This raises the exciting possibility that selected RBDs could also be specifically interacting with Rab family members therefore extending the functionality of the RBD domain.
Analysis of the binding of scFv G28-TRAIL to HeLa cellscFv G28-TRAIL0 toansfectants as well as to HeLa80 and HT1080-cellstrandfectants by FACS further demonstrated the functionalitransfectantsv domasn.
Binding kinetics and the dissociation constants for both Fcγ receptors (Fcγ RI/CD64: ~0.2 nM; Fcγ RIIIA/CD16a: ~60nM) were not compromised by PTS, demonstrating the full functionality of the Fc domain (see Additional file 1).
The TAp73α variant has a lower transactivation potential than the β isoform [ 101, 102, 109] that lacks exon 13, leading to a loss of functionality of the SAM domain [ 97, 99, 111].
Then, to learn if Cu actually impedes functionality of the SBP domain, transcript levels of selected SPL7-targets were monitored by qPCR in 7-day-old wild-type and GFP::SBP/ spl7-2 seedlingrownonn on a gradient of Cu concentrations.
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