Sentence examples for functionality of the constructed from inspiring English sources

The functionality of the constructed EHT was confirmed first using electron microscopy, showing fully developed adult cardiomyocytes with normally arranged sarcomeres (Fig 2A).

Given the crucial role of mechanical stimuli for proper cell function, controlling stresses in tissue engineering constructs and mimicking the mechanical environment of the cells can increase the functionality of the construct.

Functionality of the construct, pAKEnt (plasmid containing pfeA) and pAKFpvA (plasmid harbouring fpvA), was checked by transforming it in P. aeruginosa K407Δ pfeA and P. aeruginosa K437Δ fpvA, respectively and monitoring phenotypic complementation.

This polycistronic construct allowed the rapid monitoring of the functionality of the construct by direct visualization of GFP expression by fluorescence microscopy (Additional file 1: Figure S1B).

The functionality of the construct was achieved with short interfering RNA (siRNA) and the tumor-targeting peptide, chlorotoxin (CTx), to improve tumor specificity and potency.

To confirm the functionality of the construct and its ability to express in dicotyledonous plants in the targeted organelle, we used Western blot analysis of transiently transformed tobacco lines prior to stable transformation, and showed that the codon-optimized faeB genes were expressed in the apoplast, endoplasmic reticulum and vacuole with expression being highest in the endoplasmic reticulum.

The functionality of this construct was confirmed by testing its ability to suppress the growth defect of IY28 cells.

The functionality of this construct was verified by transfection.

HEK-293T cells transfected with wild-type and tumor-derived FAS pKEX constructs were assayed for exogenous FAS expression, in order to see both the proper functionality of the constructs and the possible variations in the expression levels among samples.

In order to validate the functionality of the constructs and to confirm that the fluorescently tagged KChIPs behaved as expected for the wild-type proteins, we assessed their expression in cell lines and their ability to stimulate the traffic of Kv4.2 to the plasma membrane based on imaging of expressed Kv4.2 [ 15].

At 20 °C, we observe that complementation of elf3-4 with either transgenic allele (Pro Bay-0 : ELF3 Bay-0 ; Pro Sha : ELF3 Sha ; Pro Bay-0 : ELF3 Bay(A362V) ) restores wild-type PIF4 levels (Fig.  4a), demonstrating functionality of the constructs, but suggesting that allelic differences are absent at 20 °C.