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"The major modeling insight we had a few years ago was to break up the functionality of the cell into subgroups, which we could model individually, each with its own mathematics, and then to integrate these submodels together into a whole," Dr. Covert said.
Traditionally, the approach is to immobilize cells to utilize a specific functionality of the cell in the system.
However, signatures seem to provide only an incomplete representation of the functionality of the cell.
These mutations are random and sufficiently rare such that they usually do not disrupt the functionality of the cell.
This fact, in combination with the study of enrichment in functional modules (GO, KEGG and Biocarta) suggests that co-expression modules are capturing the functionality of the cell.
This suggests that co-expression modules are capturing part of the functionality of the cell while signatures fail to do so at the same extent.
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The functionality of the cells was evaluated by incubating the cells with acetylated low-density lipoprotein (DiI-Ac-LDL; Biomedical Technologies, Stroughton, USA).
We measured PI exclusion and found DKO MEFs to be sensitive to BE/cholesterol, as a control for the functionality of the cells etoposide was included (Figure 5A).
Further studies of the ultrastructure of the ACROSIN-positive cells via electron microscopy will aid in diagnosing the functionality of the cells.
Besides the altered functionality of the cells themselves, detrimental effects of senescent cells might be due to an altered secretion phenotype.
The isolation of monocytes and their tissue culture plates might affect the functionality of the cells and modify them relative to their in vivo activation state.
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