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Although we did not confirm the functionality of the candidate pancRNAs, we refer to these transcripts as a fraction of ncRNAs based on their lower coding potential as explained later (Additional file 2: Figure S5A, B).
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Transgenic experiments are under way to verify the functionalities of the candidate genes.
Given that both Drosophila and human Bora can be phosphorylated by Cdk1 (Hutterer et al. 2006; ESM Fig. S 1B and S 1C) and a potential Cdk1-induced PBD-docking site in hBora (S252) has been conserved in evolution (Fig. 4e), we tested the functionality of this candidate PBD docking site in a far-Western ligand-binding assay (Neef et al. 2003).
As a final step, we used Alamut version 1.5 software to evaluate, in silico, how non-synonymous variants would affect the functionality of the respective candidate proteins (Figure 1).
A major focus of our efforts has been the development of ranking functionality that combines the candidate document information retrieved by the various text mining-based search systems into a list ordered in a way where documents most likely to be relevant to the query reaction are presented first.
Unfortunately, it is currently difficult to evaluate the functionality of candidate signalling molecules expressed during eyespot development.
Nicholas Katsanis (Duke University, USA) presented an elegant approach to resolve the functionality of candidate genes and variants using morpholino-induced translation suppression in zebrafish.
Before committing to synthesis of tagged primers, the functionality of candidate multiplex primer panels is examined in a series of amplification reactions that use prototype templates representing individual microbial targets.
Biologic inference on functionality of individual candidate single nucleotide polymorphisms (SNPs) within genes of interest may be limited and conflicting, and so choosing multiple genes in related pathways is attractive.
The methods constitute a framework to interpret functionality of GWAS loci candidate regulatory SNPs based on epigenetics, regulatory potential and TF binding affinity properties.
Furthermore, the malaria vaccine candidate AMA1 provides the possibility of assessing functionality of the immune response by a parasite growth inhibition assay.
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