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Human TAp63α transfected in human SAOS-2 cells was analyzed in parallel to verify the functionality of the assay.
Functionality of the assay was confirmed with the known proteasome inhibitor lactacystin).
Nonetheless, we believe it is adequate for demonstrating the functionality of the assay as an initial proof-of-concept phase.
Primers for α-tubulin were included to assess RNA integrity and demonstrate functionality of the assay [ 33].
The functionality of the assay in cell cultures without ultrasonic treatment is attributed to the high cell-membrane permeability of coelenterazine.
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If ConA behaves in a similar manner, the functionality of the assays would be affected as the availability of active receptors within the capsules may decrease.
The ability of VP6-specific antibodies to inhibit RV infection was studied in vitro by an ELISA-based RV antigen reduction neutralization assay., Functionality of the neutralization assay against RV Wa and RRV was confirmed using human RV-positive and RV-negative sera (data not shown).
In addition, fluorescent responses to varying glucose concentrations, leaching studies, and long-term functionality of the encapsulated assay are demonstrated.
Primers for α-tubulin were included to assess RNA integrity and demonstrate functionality of the RT-PCR assay [ 33].
When using 10 µM β-mercaptoethanol in PBS or PBS alone, two potent ADAM17 inhibitors, we were able to inhibit its activity, confirming the functionality of our assay (Fig. 4D).
The functionality of the displayed scaffoldin was then assayed.
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