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Nevertheless, considerable basic research is still required to ensure the specific differentiation, long-term fate and functionality of stem cells after transplantation.
The in vivo study of adult stem cells indicated that adequate telomere length is a prerequisite for the functionality of stem cells (Flores et al., 2008).
Thus, one can envision a time at which EEG, fMRI and evoked potentials will be utilized to assess functionality of stem cell-derived neuronal grafts in humans.
Accumulated abnormal DNA methylation during a lifespan is believed to have an impact on the behavior and functionality of stem cells [ 89].
There are many evidences indicating that aging has a clear impact both in the numbers and in the functionality of stem cells [ 7- 12] and B lymphoid progenitors [ 13- 15].
The knowledge deriving from this study will be used in the future to design an in vitro environment that mostly resemble that of the physiological stem cell niche, able to preserve the integrity and functionality of stem cells in vivo.
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In her doctoral work she studied the in vivo behaviour and functionality of mesenchymal stem cells as vehicles for gene therapy of tumours and for tissue regeneration using bioluminescence imaging.
Systematic studies investigating how fibrillar and oligomeric forms of Aβ influence the differentiation and functionality of human stem cells are therefore important.
However, with increasing age the functionality of adult stem cells declines and genomic mutations accumulate.
It is conceivable that these alterations affect the functionality of both stem and progenitor cells.
In this ex vivo culture system, organoid-forming ability is directly related to the functionality of Lgr5+ stem cells and niche-supporting Paneth cells (Durand et al., 2012, Sato et al., 2011).
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