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Since, it is thought that SCs secrete a number of NTFs comprising of NFG, BDNF, and GDNF which act through different mechanisms to enhance and accomplish complete nerve regeneration, the incorporation of SCs may be considered a valuable addition in enhancing the functionality of nerve conduits [ 154, 155, 160].
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The biggest hurdle is the functionality of it.
Previous attempts to innervate in vitro 3D skin models did not demonstrate an effective functionality of the nerve network.
The neurogenic inflammation level of the healing process is directly related to the amount and the functionality of cutaneous nerve fibers.
This is an interesting difference between muscles that could be a consequence of the different innervation with either trigeminal branches (masseter muscle) or spinal branches (tibialis muscle) and functionality of the nerves regarding motor and sensory functions [58].
Our findings are of great importance because a highly interdependent relationship between Schwann cells and axons is required to maintain proper integrity and functionality of peripheral nerves throughout lifetime.
The aim of our research has been to evaluate the functionality of the optic nerve and retinal ganglion cells by ocular examination in subjects affected by CF. The study protocol was approved by the Ethics Committees, and in accordance with Helsinki Declaration written consent was obtained for each subject or their parents.
Full recovery of nerve functionality could ideally be achieved by proper guiding axon regeneration toward the original target tissues, through the use of purposely engineered artificial nerve guidance channels (NGCs).
Neural tissue complexity, donor-site morbidity, and limited tissue availability in autologous tissue grafts result in loss of nerve functionality that cannot be restored using surgical methods alone; often resulting in the recruitment of surrounding nerves to compensate for the damaged tissue.
It is also possible that chronic inflammation may enhance the functionality of afferent cough nerves at the level of the TRPV1 receptor.
In regards to tetraplegic patients who have inadequate functionality of the phrenic nerves and in whom the implantation of phrenic/diaphragmatic pacemakers is not possible, there are experimental studies in animals that attempt to restore ventilation without respirator with high-frequency spinal cord stimulation (HF-SCS) of the intercostal muscles.
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