Exact(4)
Genes of the Argonaute (AGO) and Dicer-like family are encoded by Trichomonas and hence suggest the existence of functional RNA interference mechanisms [ 5, 14], although other studies question the functionality of identified miRNAs in this parasite [ 19].
We confirm functionality of identified eQTLs through their significant enrichment within open chromatin and highly deleterious SNPs, particularly at the exon level, suggesting substantial contribution of eQTLs to alternative splicing regulation.
To bioinformatically assess the functionality of identified cis-sequences, a new web tool was developed that can be accessed online at http://www.pathoplant.de/expression_analysis.php.
Therefore, the only applicable approach for QTL identification and candidate gene detection in large farm animals is the combination of different pieces of evidence supporting the functionality of identified genomic regions in relation to multigenic traits (Mackay 2004).
Similar(56)
Usually, the functionality of new identified cell wall genes in grasses can only be inferred from the information available from Arabidopsis or rice based on sequence homology searches.
The cognate binding motif for NF-κB was significantly enriched in the regulatory regions of consolidation-associated genes from hippocampal area CA1 compared to randomly selected genes.7 Which genes contained regulatory regions for NF-κB, out of their 38 plasticity-associated genes from hippocampal area CA1, as well as the functionality of the identified κB-sites, remain to be detailed.
Further work is required to address the role of HIF in their induction by hypoxia and to determine the functionality of the identified HREs.
In order to test the functionality of the identified NLS sequence, we used a pNIA vector-based genetic one-hybrid system that allows identification of nuclear proteins in yeast cells [39].
Next, we tested the functionality of the identified p53REs.
By using inducible recombinant expressions systems the functionality of four identified qnr candidates were evaluated in Escherichia coli.
Starting from the described results, further in vivo experiments (mass spectromic validation and genetic experiments) should be carried out, testing activity and functionality of the identified peptide products.
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