In a side-by-side comparison, we document that a C-terminal GFP tag impairs Cse4 functionality, causing severe growth defects and substantial extra-centromeric accumulation.
Hence, this truncation affects the enzyme functionality causing a severe phenotype.
In addition, the presence of a protein cannot be implied to indicate its functionality as pathogenicity can be caused by functional problems not affecting the stability of the protein [Mangold et al., 2005; Raevaara et al., 2005].
This notion is further supported by the DAVID-based predictions of alterations in cellular (KEGG) signaling pathways significant for islet functionality that are potentially caused by the differential gene expression occurring in the RasGrf1 KO islets.
The unique sensitivity of TGCT cells to Cisplatin has also been linked to an extensive and rapid induction of apoptosis and to a reduced ability to induce cell cycle arrest, probably caused by altered functionality of the p53 pathway in these cells [12].
This strategy is competitive with a 4-pathway genomic breeding program if the fraction of selected male calves for the artificial insemination program is below 1% and if selection is focused on functionality, thus pointing to substantial insufficiencies caused by low reliabilities of breeding values for cows for such traits in conventional bull dam selection schemes.
It has been suggested previously [19], that expression and localization of ClC-7 is normal in ADO II patients implying that this pathology is caused by a reduced functionality of G213R ClC-7.
The same authors have suggested that expression and localization of ClC-7 is normal in ADO II patients implying that this pathology is caused by a reduced functionality of G215R ClC-7.
However, we can not exclude that reduced functionality of the transporter could be caused by an altered intestinal cell pH, originating from LAB metabolism inside the worm gut that leads to an increased mRNA level as a compensatory mechanism.
