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However, several challenges to this technology remain before it is ready for wider use in preclinical research, including high cost, inefficiency in producing mature cell types with realistic functionality, and difficulty developing cultures enriched with mature (desired) cells and without undifferentiated (undesired) cell types that retain the potential for tumor formation in vivo.
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However, the use of human bone tissue is associated with practical issues such as availability of tissue, donor-related variability and difficulty in maintaining the functionality and viability of the implant (Holzapfel et al., 2013; Hutmacher et al., 2009).
In terms of the assignment in ECE 4760, we believe what our project lacked in functionality and user friendliness come demo time was made up for by the complexity and difficulty in our high level design.
Pain and difficulty swallowing.
Pick a character to play and difficulty.
The alternative of separating the pilot into areas or GP practices was also rejected on practical grounds because of the difficulty of matching patients on morbidity, functionality and socio-economic criteria without encountering the same ethical objections as before [ 1].
Without intervention, anemia can lead to significant symptoms like fatigue, breathlessness, difficulty in concentration and other effects on functionality and quality of life therefore treatment of anemia in HIV should be aimed at correcting the underlying cause [ 16].
Very expensive relative to scale economies of batch production; Need to attach high value to customised products; Technical difficulty in producing all but simple parts; Limited range of materials used – constrains functionality; and.
On the other hand, drawbacks include the difficulty of predicting transcription factor binding in mammals, lack of knowledge of gene functionality and regulation, and the inability to measure post-transcriptional regulation.
However, this strategy is limited by the difficulty of identifying optimal amounts and combinations of defined factors, preserving their biological functionality and releasing them according to suitable kinetic profiles.
"I'm big on functionality and durability.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com