Sentence examples for functional transposition from inspiring English sources

In addition, the in vitro transposition technology and the ability to electroporate functional transposition complexes extended the types of organisms that could be analyzed beyond those that are the standard subjects of molecular geneticists.

The plasmid vehicle strategy requires a functional transposition system, either native or of heterologous origin.

One obvious possibility is that these regions are functional transposition sequences, and would be the equivalent to the short TIRs of the P-element.

Consequently, the transfection of piggyBac mRNA by PEI enabled in vitro and cellular translation of a functional transposase, permitting transposition events to occur with high efficiency.

Each class contain elements that encode functional products required for transposition (autonomous) and elements that only retain the cis sequences necessary for recognition by the transposition machinery (non-autonomous).

Simple DNA transposition and functional gene relocation from the extant organellar genomes have both been demonstrated experimentally (Thorsness and Fox 1990; Huang et al. 2003; Stegemann et al. 2003) and found to occur at previously unexpectedly high frequencies.

Also considering the diversity of ORFs interrupted by IS 6110 copies, gene knock out studies and assessment of downstream gene expression are necessary if a functional role for specific transposition events is to be established.

Tc1/mariner family transposon termini comprise at least three types of functional sequences involved in transposition: the 4 7 nucleotide TE cleavage sites at the outer extremities of the TIRs, the DRs within the TIRs, which are the transposase binding sites, and the UTRs, between the TIRs and ORFs, which act as enhancers of transposition efficiency [ 49, 57- 59].

Thus, multifractality may have occurred extensively in the past by the apparent "over-transposition" of different functional units (Alus, CGI) carried by each DNA sequence.

This suggested that retroviruses that remained functional were initially selected and that transposition by reinfection was relevant, while loss of function would have likely resulted in neutral evolution within the host genome ω ≈ 1.

Yet, SINE transcription may take place without subsequent transposition, and the known functional effects of mammalian SINE transcription [ 57- 59] and the recently reported SINE RNA toxicity [ 60] suggest both active SINE transcription in later development, and the possible need for regulation.