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With the wealth of genomic data available it has become increasingly important to assign putative protein function through functional transfer between orthologs.
Phylogenetic profiling also helps in structure-based functional transfer: similar structures do not ensure similar functions (Devos and Valencia, 2000, 2001).
The CAFA experiment provides evidence that the best algorithms universally outperform simple functional transfer.
The temporalis muscle is commonly used for functional transfer.
The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage.
Following amputation, the defects were covered with an ipsilateral pedicled latissimus dorsi (LD) myocutaneous flap for stump protection and functional transfer.
The successful functional transfer of an N-glycosylation pathway from Campylobacter jejuni to Escherichia coli in 2002 can be considered as the crucial first engineering step.
Trimethyl and vinyl α,ω-terminated PDMS oligomers were similarly synthesized in miniemulsion in the presence of functional transfer agents and used together with small dihydroxylated PDMS standards to compare the effect of substituents on the SEC calibration curve.
These GO terms are used for functional transfer and annotation.
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However, the timing of functional transfers has been unclear.
Functional transfers of P-gp have also been described very recently between leukemic cells cultured in suspension [ 25].
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