In an attempt to identify other drugs that inhibit the function of AQP4 in water transport, Mola et al [15] developed a functional high-throughput assay based on the measurement of osmotically-induced cell volume changes to screen several libraries of drugs and identified four blockers of AQP4-mediated water transport.
To rapidly identify the residues within the Kv1.5 channel critical for blocking activity of these molecules, two functional high-throughput ion channel assays were employed together with site-directed mutagenesis.
Functional high-throughput screening of effector gene elicitation of the HR in plants was performed using a Potato virus X (PVX) agroinfection assay optimized for Solanum [13].
Thus, our robot extracts ultra-pure plasmid DNA from the complex mixture of bacterial lysates and makes possible functional high-throughput screens based on iterative cell transfections.
The described Cpn ORFeome is the basis for functional high-throughput analysis of this organism, providing a flexible tool for quick and efficient genome-wide examination and discovery.
Moreover, since many gene activities cannot be assayed in selections and testing gene pools is often not sensitive enough, functional high-throughput screens of individual genes are required.
Our laboratory performed a functional high-throughput screen (HTS) (18− 20) that led to the identification of about 20 structural classes of mGlu5 PAMs.
These results show that the wheat cell-free protein expression system and the luminescent ubiquitination detection method could support functional high-throughput screening of E3 proteins.
Thus, we performed a functional high-throughput screen (HTS) and identified a novel mGlu5 PAM benzamide scaffold, exemplified by VU0001850 (EC50 = 1.3 μM, 106% Glumax) and VU0040237 (EC50 = 350 nM, 84% Glu Max).
Here we reveal the construction and use of this custom-made robot for the isolation of ultra-pure plasmid DNA for efficient transfections that can be used for many additional functional high-throughput screens.
