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Finally, a functional test was performed by measuring the peak amplitude of the delayed potassium current in the −30/−10 mV range, over which the voltage dependence of the I V curve becomes steep.
For those urine samples positive for XA, a parallel determination of activation coefficients on RBC's aspartic acid aminotransferase (AST /B-6 functionAST /B-6was perfunctionalconfirm if XA-positest individuals wase definitively deficient in vitamin B6.
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In vitro muscle contractile functional testing was performed in an organ chamber with electrical stimulation.
Functional testing was performed in vitro by plasmid transfection followed by PDGF-AL and β-galacatosidase (β-gal) protein detection (data not shown).
All the functional tests were performed at the same age as the cohort treated with 5% ribitol from 7 week old for 3 months and age-matched untreated controls.
Functional tests were performed on the RSU as mentioned above, but other aspects also needed to be tested.
Neurological functional tests were performed by an examiner blinded to the treatment status of each animal.
Functional tests were performed and the infarct volume was measured at 7 and 14 days after MCAO.
Functional tests were performed at 1, 4, 7, 10 and 14 days after MCAO by the modified neurological severity scores (mNSS).
Next, functional tests were performed by disaggregating EBs and seeding 5,000 cells/well in a 384 MTP under the same differentiation conditions.
The functional tests are performed by a single-blinded researcher or other single-blinded qualified personnel.
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