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This is the first demonstration of human NoV P domain protein as a functional target for the selection of nucleic acid aptamers that specifically bind and broadly recognize diverse human NoV strains.
It has been reported that spermatogenesis appears to be the functional target for newly evolved genes in the lineages of Drosophila.
This result further supports FAK as a functional target for reversine.
Moreover, the mitochondrial respiratory chain complex I was recently reported to be a functional target for NOX4 (8).
Also the miR-372 was identified as a direct and functional target for ATAD2 in hepatic carcinogenesis.
Either EMSA or DNase I footprinting is required to determine whether it is a functional target for PhoP-specific binding.
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Another study used a combination of experimental data and computational analysis to predict functional targets for miRNAs in the inner ear (Elkan-Miller et al, 2011).
Currently, miRNA target identification is primarily based on computational predications that could result in a vast number of targets with errors, raising the problem of identifying functional targets for validation [ 42].
To obtain high confidence functional targets for each of the miRNAs, we set a cutoff of two median absolute deviations (mad) from the median prediction score of all miRNA-mRNA interactions.
Moreover, functional targets for SRSF1 are more diverse than optimal binding sites, since half of them have neither a GGA nor a GAA motif (Liu et al., 1998a; Smith et al., 2006).
Furthermore, we have searched for the functional target of the key miRNA using computational analyses.
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