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The resulting constructs are functional, stably maintained under conditions where the original constructs are unstable, completely defective for conjugative transfer, and transferred via electroporation.
Some of the limitations anticipated by these two latter issues should be overcome by induced pluripotent stem (iPS) or induced stem (iS) cells, which both provide an alternative source of functional, stably expandable [ 71, 201], and therapeutically efficacious MSCs or NPCs for tissue repair [ 202, 203].
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Taken together, we conclude that GAA is not stably functional and the GFP insert separating the entire N-terminus from TM might have given an altered feature to the GCS1 molecule.
This work shows that the Cas9 system is functional in two stably transformed plant systems, hairy roots and somatic embryos.
Iron oxide nanoparticles were incorporated into the polymer matrix using common photolithographic techniques to create stably functional microstructures with magnetic potential.
From the data presented here, clearly the DNA segments tested, representing sequences from proteins with significant inherent functional differences, were stably incorporated into the gvpC gene.
Theoretically, by modifying some functional genes, we can stably enhance yield and even improve the properties against bacteria or diseases (Gan and Maggs 2017).
To analyze the role of adhesion-mediating β1-integrins, stably transfected functional β1A-integrin-expressing GD25β1A and GD25β1B cells, which express mutant β1B-integrins, were compared in terms of radiation survival and β1-integrin signaling.
Functional proteins can be stably incorporated into supported membranes, and these can form signaling interfaces with living cells.
For this purpose, we have used splenic injection model of liver metastasis with colon adenocarcinoma-derived FET cells that stably express functional Smad7 (Halder et al, 2005).
Specifically, mutations in certain kinetochore proteins were shown to abolish de novo establishment of the S. cerevisiae centromere, although functional centromeres could be stably propagated for over 25 generations in this background (Mythreye and Bloom, 2003).
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