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The development of cell-sheet tissue engineering, where cells are plated and allowed to form confluent layers which are then dissociated from the plate to form intact, functional sheets, has generated a need for a systematic characterization of cell-cell interactions to better condition constructs for in vivo use [ 1- 3].
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The present study describes an approach to generate a functional sheet of pancreatic islet cells on laminin-5 coated temperature-responsive dishes, which can be subsequently transplanted in vivo.
As a next generation of cell sheet-based tissue engineering, extracellular matrix-mimicked thermoresponsive cell culture surfaces are required for creating functional cell sheets.
Furthermore, β-cyclodextrin functional graphene sheets (CD-GS) were used as matrixes for immobilization of antibodies due to the huge surface area and superior electrical conductivity.
A heparin-modified thermoresponsive surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was designed to allow creation of transferrable and functional hepatocyte sheets.
Aged cells showed an impaired ability to form functional cell sheets, as determined by morphological observations and Ki-67 immunohistochemistry staining.
This approach involves the direct growth of thermoresponsive polymer brushes from functional graphene sheets (FGSs) by chemical modification with initiators followed by extension with poly 2- dimethylamino)ethyl methacrylate) (poly 2- dimethylaminoacethyltiated atomethacrylateadical PDMAEMAizathrough
A nanocomposite composition having a silicone elastomer matrix having therein a filler loading of greater than 0.05 wt %, based on total nanocomposite weight, wherein the filler is functional graphene sheets (FGS) having a surface area of from 300 m2/g to 2630 m2/g; and a method for producing the nanocomposite and uses thereof.
Generally, epithelial cells must organize in three dimensions to form functional tissue sheets.
Finally, we used UV spectroscopy and TEM to design a metric that may be suitable for rapid characterisation of functional graphene sheet size after exfoliation step.
In this study, an interpenetrating network of the electrically-conductive polymer polypyrrole (PPy) was grown within a matrix of flexible polycaprolactone (PCL) and evaluated as a platform for directing the formation of functional cardiac cell sheets.
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