The development of cell-sheet tissue engineering, where cells are plated and allowed to form confluent layers which are then dissociated from the plate to form intact, functional sheets, has generated a need for a systematic characterization of cell-cell interactions to better condition constructs for in vivo use [ 1- 3].
Hence, heparin-modified thermoresponsive surfaces bound with HB-EGF facilitate the fabrication of transferrable hepatocyte sheets with intact hepatic functions and have the potential to provide an in vitro culture system using functional hepatocyte sheet tissues, which may serve as an effective hepatocyte-based tissue engineering platform for liver disease treatments.
Finally, we used UV spectroscopy and TEM to design a metric that may be suitable for rapid characterisation of functional graphene sheet size after exfoliation step.
Using fabricated cell sheets harvested from temperature-responsive surfaces including deposited extracellular matrices, we have established cell-sheet technology to fabricate functional tissue sheets to treat a wide range of diseases from corneal dysfunction to esophageal cancer, periodontal disease, cartilage disease, and cardiac failure.
As a next generation of cell sheet-based tissue engineering, extracellular matrix-mimicked thermoresponsive cell culture surfaces are required for creating functional cell sheets.
Furthermore, β-cyclodextrin functional graphene sheets (CD-GS) were used as matrixes for immobilization of antibodies due to the huge surface area and superior electrical conductivity.
A heparin-modified thermoresponsive surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was designed to allow creation of transferrable and functional hepatocyte sheets.
Aged cells showed an impaired ability to form functional cell sheets, as determined by morphological observations and Ki-67 immunohistochemistry staining.
This approach involves the direct growth of thermoresponsive polymer brushes from functional graphene sheets (FGSs) by chemical modification with initiators followed by extension with poly 2- dimethylamino)ethyl methacrylate) (poly 2- dimethylaminoacethyltiated atomethacrylateadical PDMAEMAizathrough
Generally, epithelial cells must organize in three dimensions to form functional tissue sheets.
