These methods are followed by antibiotic selection of a stable pool of cells and functional screening to identify individual clones that have the correct function(s).
Fortunately, a variety of novel high-throughput genome-wide functional screening technologies provide important clues that shed light on gene function.
In summary, functional screening of the metagenomic library revealed a protease positive clone.
We established a novel functional screening system for peptides acting on G-protein coupled receptors (GPCRs).
Figure 1 Functional screening of metagenomic library for protease activity on skim milk agar plate.
Directed molecular evolution is a recursive process of controlled genetic diversification and functional screening.
All six clones that expressed a cellulase in initial functional screening were tested for their thermal stability.
In this study, we established the novel functional screening system of yeast-secreted peptides acting on GLP1R.
However, functional screening in human monocyte-derived macrophages using the xCELLigence label-free platform demonstrated altered specificity of our ligands.
More recently, Zhou et al. carried out a small-scale functional screening and identified that loss of Bmi1 significantly promoted mouse cardiac reprogramming.
The six cellulase expressing fosmid clones identified by functional screening were used to prepare sub-libraries with Nextera bar-codes and pooled for sequencing using Illumina MiSeq.
