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In recent years, purification and functional reconstitution of the protein has allowed the application of fluorescence spectroscopic techniques.
The functional reconstitution and the formation of a conducting pore are shown by electrochemical impedance spectroscopy (EIS).
Tissue engineering (TE) approaches typically envisage the structural and functional reconstitution of previously damaged tissue in situ.
The ligation step in PTS must be performed under conditions compatible with protein folding because the process involves the functional reconstitution of a split intein.
Our work thus provides a useful approach for functional reconstitution and engineering of fungal CODSs for efficient production of this family of anticancer molecules.
Functional reconstitution of porins into the capsule shell allows to control the rate and selectivity of substrate permeation, and thus to control the enzyme reaction kinetics.
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Functional immune reconstitution seems essential for the antileukemic activity of lenalidomide in CLL [ 8].
Furthermore, the multiple components and complex topology of ER-resident ligases (Hirsch et al., 2009) has thus far precluded their functional membrane reconstitution.
Although complete functional immune reconstitution is rarely achieved, the beneficial effect of HAART in HIV-infected adults [ 16- 19] and children [ 20- 23] is well documented.
It now appears most likely that genome reduction, probably by intrachromosomal recombination and deletion events, led to stochastic fragmentation of multiple genes, with those split in locations compatible with functional enzymatic reconstitution in trans being retained.
We have used functional co-reconstitution of purified sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) with phospholamban (PLB), its inhibitor in the heart, to test the hypothesis that loss-of-function (LOF) PLB mutants (PLBM) can compete with wild-type PLB (PLBW) to relieve SERCA inhibition.
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