Following induction of sepsis by CLP, a marked elevation in serum levels of AST and ALT, a functional readout for liver damage, was observed in WT mice (Fig. 5a).
Moreover, the combination of sensitive brain imaging techniques and metabolomics platforms hold optimism to identify novel metabolite-gene relationships and new insight into small molecular changes in neuronal metabolism to enhance our understanding of biochemical pathways "functional readout".
Aside from EEG as a functional readout, we found that propofol treatment protected the auditory brainstem response (ABR) threshold as compared to isoflurane anesthesia in this model as published previously [25].
The comparative analysis of both structurally and functionally of protein primary, secondary, and tertiary structure generates numerous hypotheses to probe the relationship between molecular structure and the ensuing functional readout.
Thus, polyp formation serves as a functional readout of genomic instability.
The potential to regulate transcription is one functional readout of such binding.
A well-defined abasic bypass reporter system [23] thus can provide a functional readout for FANCD2 regulation of pol η.
The extent of cell shortening provides a functional readout of [Ca2+]i transients and contractility, since cardiomyocytes respond to elevated calcium levels by contraction.
It also provides a functional readout for ROR2 signalling although, given ignorance of the downstream signalling pathways employed by ROR2, this could indicate either attenuation or sustained signalling.
As for an additional functional readout, preadipocytes transfected with a TLR4-specific siRNA showed significantly reduced IL-6 secretion upon stimulation with LPS compared to the controls.
The development of 3D, skin-like tissues in vitro provides a functional readout of stromal cell capacity to provide cross-talk needed for epithelial morphogenesis [30], [31].
