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The conclusion supports the prediction that the antifungal activity of CkTLP is due to the functional patch composed of E83, D97, D102 and D183.
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Therefore one can compare a protein of unknown function with a set of well-characterized structures in order to check whether there are local similarities involving the known functional patches.
Thus, the findings of this study clearly demonstrated that functional cardiac patches with superior electrical and mechanical properties can be developed using nanoengineered GelMA-GNR hybrid hydrogels.
Thus, tissue engineering of functional cardiac patches critically depends on our understanding of the interaction between multiple guidance cues such as topographical, adhesive or electrical.
Here we provide design criteria for the generation of functional cardiac patches, and discuss different biomaterials and cell types used during the tissue engineering process.
The layered and patterned coculture strategy achieves the spatial arrangement of multiple types of cells and vascularization potential, providing a biomimetic strategy for engineering functional cardiac patches in vitro.
Local structural comparison methods can be used to find structural similarities involving functional protein patches such as enzyme active sites and ligand binding sites.
Thus, physiologic mechanical conditioning may be essential for generating functional cardiac patches for replacement of injured cardiac tissue.
Barash et al. [ 53] used a custom-made electrical stimulator, integrated into a perfusion bioreactor originally described in 2006 by Dvir et al. [ 70], with the purpose of producing thick and functional cardiac patches.
By providing a suitable mechanical stimulus for in vitro cardiac cultured tissues, our CCCM system holds significant promise for generation of functional cardiac patches for replacement of injured cardiac tissue.
Moreover, as we conduct the minimization of the target functional on each patch separately, we use different values for the Lagrange multiplier for each patch.
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