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Nevertheless, the observed symbiovar orientalis-specific rpoN2 gene as well as the nifQ gene, found in both symbiovars, were shown to be important for functional nitrogen fixation.
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As a further control, the same experiment was conducted in the presence of HN1 (2-dimethylaminoethylchloride hydrochloride), a mono-functional nitrogen mustard which does not form ICLs [26].
The upregulated genes mainly belonged to three different functional categories: nitrogen metabolism-related genes, including a set of nitrogen fixation-related genes; a large number of genes encoding transporter and membrane proteins; and genes with unknown function.
One interesting common trait in all the comparisons made of the transcriptional changes observed either by temperature or strains was the presence of functional categories "nitrogen, sulfur and selenium metabolism", "sulfur metabolism" and "metabolism of methionine" in the genes up-regulated at low temperature (Table 2).
However, GCN2 regulation is not thought to be functional in nitrogen-starved cells [ 52].
Redox peaks, observed during cyclic voltammetry analysis of the functionalized GAC, evidenced that these nitrogen functional groups were electrochemically active, so that they sorbed perchlorate when they were oxidized, and then desorbed perchlorate when reduced.
The existence of nitrogen functional groups on carbon nanotubes and the type of functional groups after pyrolysis was confirmed by x-ray photoelectron spectroscopy (XPS).
XPS showed that only CMK-3-NH3 had nitrogen functional groups, whereas CMK-3-H2SO4 had the largest amount of oxygen functional groups.
When mitochrondria are functional and sufficient nitrogen is available, the transcription factors are cytoplasmic; disruption of mitochrondrial function or nitrogen depletion results in nuclear localization of the factors and subsequent transcriptional activation of target genes.
Nitrogen functional groups were identified from RB and RA-afterTEM.
Schematic illustration of nitrogen functional groups in the carbon lattice (f).
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