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The number and types of functional measurements which can be determined using MR imaging are multiple.
Since good tissue viability is critical for quantitative assessment of bioelectric responses our recommendation is that specimens are maintained in appropriate medium on ice until used in functional measurements, which should be performed immediately [ 13].
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A 400-m run at maximal speed was used as a functional measurement, which we have previously used successfully [2, 3].
Strengths of the present study are the standardized setting of the test procedures and measurements according to recommendations of previous studies, the relatively (in comparison to other studies) high and a priori power-based sample size, and the combination of electrophysiological and functional measurement, which yielded consistent results.
The speed helps to minimize motion artifacts for in vivo measurements, which in addition results in better functional contrast.
Differences in continuous outcomes (pain scores and functional scores) were analysed with linear regression techniques for repeated measurements, which take the correlation of multiple measurements within one patient into account.
Automated measurements, which will become easier with every advance in digital image acquisition and analysis, provide hitherto unobtainable data with which to investigate functional, physiological, or developmental attributes of modern leaves.
Histology, however, cannot display the rate at which perfusion takes place, which the in vivo functional measurements do.
48 h-old algal cultures were diluted down to 80 μg.mL−1 dry biomass (approximately corresponding to 8 μg/mL chlorophylls) to avoid any light screen effect which would otherwise affect functional measurements.
From these evidences, one can expect that diverse metabolic functions are improved by 3D cultures and therefore they should be examined by establishing functional assays including metabolite measurement, which may provide a useful system for drug discovery research in the future.
A low magnification structural YFP image illustrates a site with brightly labeled cells (Figure 9A) of which four were selected for functional measurements (white rectangles).
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