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Functional mapping was performed using Ingenuity Pathway Analysis (IPA, Ingenuity® Systems, Redwood City, CA, http://www.ingenuity.com).com
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Functional annotation mapping was performed using the NIAID DAVID database [ 23, 45]).
In this study, functional QTL mapping was performed for a set of important wood properties in increment cores from a 17-yr-old Scots pine (Pinus sylvestris L). full-sib family with the aim of detecting wood trait QTL for general intercepts (means) and for linear slopes by increasing cambial age.
To achieve these two aims, functional QTL mapping was performed on a Scots pine (Pinus sylvestris L). full-sib family established in a single large plot in northern Sweden using a set of AFLP and SNP molecular markers paired with phenotypic assessment of the wood of multiple annual rings in increment cores or by repeated assessments on standing trees.
To identify the p17 functional region recognized by mAb MK-18 epitope mapping was performed with a series of decapeptides that completely spanned the p17 protein.
Epitope mapping was performed in two steps.
Arabidopsis tag mapping was performed with Novoalign software (Novocraft Technologies).
Mapping was performed using rmapper.
Homozygosity mapping was performed using HomozygosityMapper.
SNP mapping was performed as described [ 36].
The mapping was performed on the Pfizer High Performance Computing cluster.
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