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It allows the functional insertion of a non-inhibitory antibody in lipid bilayers, further used to anchor a soluble enzyme at the surface of the lipid membrane.
Twelve pVT1 ORFs present similarities to transposase genes or fragments of transposases, with six corresponding to potentially functional Insertion Sequences (IS).
These studies demonstrated the importance of signaling between the various tendon-to-bone cell types for generation of a functional insertion.
We interpret this result as epitope insertion in this portion of the protein causing improper maturation and processing of the protein (retention in the endoplasmic reticulum), preventing functional insertion in the membrane as determined by the absence of an immunofluorescence signal in transfected cells (data not shown) and ultimately leading to absence of detectable transport.
Interestingly, while functional insertion of the cleaved RCL does not alter the asphericity of α1AT, the values of S and Δ are unchanged, the latent conformation with its intact RCL is significantly less aspherical [Δ = 0.15, and S = 0.11 (Table S1 of the Supporting Information)].
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We identified fully functional GFP insertions and partially functional insertions that bind DNA but fail to repress the lacZ operon.
In addition, we sequenced the region of the TCF-4 gene in which functional insertions and deletions have been reported in colonic cancer [22].
TAGIT thereby faciliates the isolation of fully functional insertions of fluorescent proteins into target proteins expressed from the native chromosomal locus as well as potentially useful partially functional proteins.
We also identified differences in the number of likely functional insertions and the average effect size of an insertion depending upon the location category of the TE insertion.
We demonstrate that a model membrane protein, the potassium channel from the bacterium Mesorhizobium loti, remains functional after insertion into the planar solid-supported polymer membrane.
Our aim was to assess the early and late complication rate, and duration that the CVAPD remained functional, following insertion by interventional radiologists in patients with solid tumours.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com