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Despite the relatively low genomic sequence coverage, 1,801 contigs (9.8%) produced functional hits and detailed annotation data, with 322 functional ontology terms, categories, and keywords cumulatively identified (Table S4).
To functionally characterize the sequence content of the 56,084 blastn-aligned contigs, the assembled sequences were again mapped onto the relevant classification schemes, with 5,057 contigs (9%) producing putative functional hits and detailed annotation data, including 606 identified functional ontology terms, categories, and keywords (Table S9).
The latter comprise around half of the functional hits.
A swine oligonucleotide microarray (70 mer) containing 13,290 functional hits was used for transcript profiling.
When the cut off was set below 10%, the differences between the assembled and unassembled functional results were smaller, and in some cases the unassembled recovered more functional hits (Newbler and Celera meta).
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Shortest paths connecting the functional hit and variant genes are also displayed for additional context.
For this reason, only 14% of physicians have minimally functional HIT systems [ 35].
In HitWalker, RWR provides a measure of weighted proximity between a set of proteins associated with functional assay hits and a set of proteins containing variants.
If we restricted the search to the functional annotations, 23 hits with homologs were returned.
Two-dimensional hierarchical clustering shows the functional organization of hits identified for all four mutants we screened.
Being produced by enzymes and selected on the basis of an intracellular functional assay, SB hits explore biologically relevant chemical space with "privileged", NP-like fragments.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com