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Functional hints are generally extracted from high-throughput studies performed on S. cerevisiae.
In addition, we identified a group of novel miRNA candidates in rat cortex with functional hints.
A group of novel microRNAs with functional hints were identified, and brain-enriched expression and Dicer-dependent production of high-abundant novel microRNAs were validated.
To confirm the 3D-Jury results and to obtain additional functional hints by other methods, we also searched for motifs (signature sequences) using the CDD [ 25], COG [ 27] and InterPro [ 26] databases.
In addition to the demonstration of dynamic and stage-specific expression of a large group of known miRNAs, we identified a group of novel miRNA candidates in rat cortex with functional hints.
To get functional hints for these novel candidates, we predicted the potential target genes of these miRNAs by using TargetScan [ 37], and carried out gene ontology (GO) enrichment analysis with GOrilla of predicted target genes (Dataset S3).
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Grönholm et al. [ 83] provided a first functional hint for neuronally expressed merlin in the CNS.
Two-hybrid screens are sometimes referred to as functional screens, since interacting proteins might give a functional hint if at least one of the partners has a known functional commitment in a well understood signaling pathway.
Thus, subtle differences in enriched functional terms hinted at possible variations in underlying immune response.
In contrast to paraquat, however, this resistance is not dependent upon functional autophagy, hinting to differential pathways governing Spd-mediated resistance to different oxidative stressors.
The dependency of these two essential amino acids on other functionally uncharacterized ones hints possible functional links between both sets of amino acid sites.
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