To avoid potential steric hindrance and functional interference between the highly structured GBV-B and HCV domains, a 23-nucleotide-long spacer was inserted upstream of the HCV IRES (see Material and Methods).
Hydrophobicity, the presence/absence of some functional groups, steric hindrance and spatial arrangement all play important roles in the affinity of natural polyphenols towards plasmatic proteins [13].
A major hindrance in functional inference from genome analysis is the large proportion of annotated hypothetical proteins, uncharacterised proteins or proteins of unknown function [ 30].
The results show that the proper amount of physically absorbed water, the use of high active trichlorosilanes, and the functional groups with less steric hindrance are essential to generate MSNs with high surface coverage of monolayers.
It proposes that insulin binding to the receptor induces MMP9 to activate Neu1 which hydrolyzes α-2,3 Sialic acid in removing steric hindrance to generate a functional receptor.
In fact, CpG site 8 encompasses a transcription factor-binding site for specificity protein 1 (Sp1) that might have been disrupted due to physical hindrance by the methyl functional group found within the Sp1 DNA consensus sequence (Fig. 2f).
The pAP47 rifH-xylE fusion construct (not shown) did not produce a functional metapyrocatachase, perhaps due to steric hindrance of the fused protein or the lack of appropriate translational signals for xylE, while the pAP50 rifH-xylE operon construct with an RBS sequence dedicated to the reporter gene worked well.
As a result, when the spatial separation of the functional units is critical to avoid steric hindrance and to preserve the folding, stability and activity of each unit in the fusion proteins, rigid linkers would be selected.
Our results show that the skeleton structure of flavones, the number of hydroxyl groups, the type of functional group, conjugated system and the steric hindrance may be responsible for their different biological activity.
Polyacrylates differed in the proportion and distribution of amine, carboxyl and hydroxyl functional groups and the degree of steric hindrance present in the polymer chain, modulated by a ratio of ethyl to butyl side groups.
