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Together, seven functional groups were identified, including genes functioning in caspase-dependent apoptosis, JNK signaling and metabolism, oxidation-reduction, trafficking, ubiquitination, nuclear export and synapse activity (Table 1).
Functional selection for repair association was performed manually using the function tree of the IPA software (Qiagen), where genes under developmental and repair functional groups were identified.
Similarly, 21, 15 and 13 functional groups were identified and classified as biological processes, cellular component and molecular function for the co-downregulated DEGs, respectively.
Based on gene ontology (GO) classification, 21, 14 and 10 functional groups were identified and classified as biological process, cellular component and molecular function, for the co-upregulated DEGs, respectively.
Nitrogen functional groups were identified from RB and RA-afterTEM.
The functional groups were identified from FTIR analysis.
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The functional groups are identified depending on their corresponding peak values in the infra red (IR) spectral region.
The importance of individual genes within these functional groups was identified by evaluating the regression coefficients of the genes.
The functional group was identified using potassium bromide (KBr) and scanned in the range of 4000 400 cm−1.
Only one functional group was identified in gene functional classification analysis; this group had an enrichment score of 1.25 and contained 5 transcripts annotated as regulators of transcription, and 4 as DNA binding (Table 6 and additional file 7).
These were analysed for up and down-regulated genes both separately and together, and functional annotation groups were identified both with and without clustering (at medium stringency).
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