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Moreover, qPCR efficiency values of the assays following multiplexed RT/pre-amplification ranged from 93.8% to 104.1%, within the commonly employed functional cutoff of 90 110%.
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Defining functional impairment cutoff points is very difficult, and even assessing function in many cases is unworkable, given its significant social and gender context.
All structures were initially fully optimized using the PBESol exchange and correlation functional and a cutoff energy of 500 eV and a Γ-point sampling of the Brillouin zone, allowing all ionic and lattice degrees of freedom to relax.
We found that this particular choice of cutoff identifies functional matches for a significant proportion of proteins across two species whilst keeping multiple hits within reasonable limits.
The same is true for the KSS functional score, where the cutoff point is 30 points for the inter-observer variability and 19 points for the intra-observer variability (Liow et al. 2000).
Although the >25% phastCons cutoff for functional training data is arbitrary, only ∼5% of the human genome is conserved across species; windows that are 25% conserved according to phastCons are thus very likely to encode important functions.
The subPSEC score is an estimate of the likelihood for a given non-synonymous mutation to functionally impact on the protein, ranging from 0 (neutral) to −10 (the highest likelihood to be deleterious), and a subPSEC score below −3 is regarded as a cutoff for functional significance [ 31, 32].
The clusters obtained from clustering of the low-rank matrix are more enriched with GO functional categories at varying cutoffs (Table 2).
Vilain says he wonders if men with functional testosterone levels below the cutoff would be allowed to compete as women, even though they are men.
Anderson et al., did not reach the predetermined cutoff value for functional outcome in arthroscopic surgery either.
Next, we analyzed the predicting power of choosing an EMSE combination for functional outcome, AUROC, and calculated cutoff point.
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