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Subsequently, endogenous Ct-OATP1B3 protein expression and functional analysis were performed by Western blotting and transport assays.
To shed light on such features, immunophenotypic and functional analysis were performed in peripheral monocytes and T lymphocytes of ALS and primary progressive (PP) MS patients and healthy controls (HC).
Annotation and functional analysis were performed using Ingenuity Pathways Analysis (IPA) 7.1 software (Ingenuity® System; www.ingenuity.com), a commercial database containing manually annotated data for human protein-protein and functional interactions derived from the literature.
The conversion to human orthologs and functional analysis were performed as described above for visceral AT from DIO zebrafish.
Expression, protein extraction, and purification of LXR3 in S. cerevisiae strain CEN.PK2-1D (European S. cerevisiae Archive for Functional Analysis) were performed as described for A. niger LxrA.
Taxonomic and functional analysis were performed on assembled sequences from all samples, using the Newbler software, and automatically annotated, using the MG-RAST server, through BLAST, against the GenBank, COG, KEGG and Subsystems databases with maximum e-value cutoff of 10-5[ 47].
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Functional analysis was performed on these networks to identify the biological functions and/or diseases that were most significant to the genes in the network.
Functional subassemblies are identified, and a functional analysis is performed.
A functional analysis was performed by ectopic expression of FnAG driven by the CaMV 35S promoter in transgenic Arabidopsis.
Functional analysis was performed according to a previously described protocol [70], [71].
Functional analysis was performed on the 1.5, p<0.05 probe list using Pathway-Express [59].
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