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They also provide evidence that CRISPRi is functional in human cells, albeit with much lower efficiency compared with E. coli.
Yang has developed a similar system with much lower efficiency.
Other single nucleotide bulges were stabilized by the ligand with much lower efficiency.
This suggests that initiation events occur with much lower efficiency at these sites compared to oriP.
Methylated cytosines are converted with much lower efficiency and thus remain cytosines.
Such substrates were hydrolyzed with much lower efficiency than short acyl groups; hence, the conclusion that they are not significant TEII substrates in vivo.
Because mismatched 3′ termini are extended by Taq polymerases with much lower efficiency than correctly matched termini, the allele-specific primer preferentially amplified the specific allele.
Therefore, to reduce the oncogenic potential of iPSCs, c-Myc can be excluded from the reprogramming cocktail but with much lower efficiency [ 37, 44– 44].
The fact that the G274A polymorphism was also found in thyroid cancers suggests that the RNA editing could be also induced by other tumours, although with much lower efficiency.
This fact and their reported capability to degrade non-phenolic lignin model dimers, although with much lower efficiency than white-rot fungal LiPs (lignin peroxidases) [ 8], suggest a possible contribution of fungal DyPs to lignin biodegradation.
However, it functions at a much lower efficiency than EMCV IRES or CrPV IRES.
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