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DNA transposons, RNA interference and CRISPR/Cas9-based systems have been powerful tools for loss-of function screening.
To compare the Nexfin cardiac output (CO) with the CO obtained from transthoracic Doppler echocardiography (TTE) during routine cardiac function screening.
Subcellular location of protein is constructive information in determining its function, screening for drug candidates, vaccine design, annotation of gene products and in selecting relevant proteins for further studies.
Consequently, it has been recommended that all adults diagnosed as having meningitis should undergo pituitary function screening.
This review focuses on several noninvasive methods that have been developed for pulmonary function screening, which are analyzed from an engineering systems perspective.
Genome-wide loss of function screening in the diploid mammalian genome is hindered by the requirement for homozygosity.
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By using an RNAi-mediated loss-of-function screening approach, we have identified CSNK1E, a member of the casein kinase family, as an essential regulator of β-catenin activity and proliferation in breast cancer cell lines.
Interestingly, in a loss-of-function screening system based on intracellular expression of single-domain antibodies, Inoue et al (2007) found that hnRNP K is a potential target for metastasis and its cytoplasmic accumulation may mediate its role in cell migration and metastasis.
Such a rapid and simple gain of function screen proves an extremely useful tool for dissecting pathogens displaying functional redundancy of multiple virulence factors and toxins.
Unfortunately, the prettiness of the home screen disappears when you enter almost any application or function screen.
Systematic gain or loss of function screens are discovering more miRNAs playing important roles in ESCs and during reprogramming (Judson et al., 2013; Wang et al., 2013b).
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