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This notion is also playing out in discussions in literature not explicitly concerned with decoding periostin function on the sequence or domain level.
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The reverse strand binding sites for the two transcription factors were similarly identified using the "Find" function on the sequences complementary to the sites.
Proper functioning of the sequence is demonstrated on a nitroxide biradical.
The candidate final cumulative incidences are assigned a likelihood function depending on the sequence of positivity of the samples in each age group by using Equation 2. As the prior distributions of the cumulative incidences are uniform over [0,1], to find the posterior distribution of the incidences, the likelihood function is renormalized so that it sums to one.
SIFT provides the tolerance index score ranging from 0 (deleterious) to 1 (neutral) of a particular amino acid substitution to protein function based on the sequence alignments.
Out of 43,990 genes de novo assembled, 34,192 unigenes were annotated their function based on the sequence similarity search against the public databases.
This could be an example of how a function assignment based on the sequence and structure similarity (InterproScan) could actually be different from a function assignment based not only on the structure but also on the physical/chemical and functional biological properties (Protfun).
Therefore, obtaining the full collection of prototypes with elementary functions will make it possible to (i) predict enzymatic functions based on the sequences via determining EFLs corresponding to prototypes and their relative positions revealing structure of the fold; (ii) (re design folds with desired functions by building constructs from necessary EFLs.
However, the modulation of substrate specificity makes it difficult to define the physiological function based only on the sequence.
SIFT is a program that predicts the effect of amino acid substitutions on protein function, on the basis of sequence conservation during evolution and the nature of the amino acids substituted in a gene of interest.
BlastP analysis [ 65] against the NCBI protein database [ 63], as well as annotation of the LPP-1 CDS using the GenDB pipeline [ 66], were used to predict their function on the basis of sequence similarity to characterized proteins.
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