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Therefore, further investigations on function of two unigenes and stilbene metabolites were needed for the identification of these genes.
Similar(59)
Among the five unigenes involved in the mitochondrial respiratory chain, the molecular functions of three unigenes (TCONS_00050124, TCONS_00134261 and TCONS_00070653) was related to cytochrome P450 activity.
For KORs, two unigenes of comp135170_c0 and comp10758_c0 were identified.
To classify the predicted function of all unigenes, Blast2GO software was employed to search against GO database and KOG database.
The function of assembled unigenes was further evaluated using Clusters of Orthologous Groups (COG) analysis.
A total of 49 836 (45.9%) all-unigenes were clustered by at least two unigenes, with a maximum of 31 unigenes per all-unigenes, whereas the rest 58 762 (54.1%) were corresponding to single unigene from any of the four sub-transcriptomes.
Total of 63, 452 all-unigenes were clustered by at least two unigenes, with a maximum of 27 unigenes per all-unigene, and the rest 212, 569 were corresponding to single unigenes.
To further evaluate the function of the assembled unigenes, we searched the annotated unigenes involved in COG.
After examining the gene annotation information available for these unigenes, it was found that information on gene function was available for three unigenes; however, no matches in any database were obtained for the remaining 8 unigenes.
Many genes involved with the plasma membrane (12 unigenes), organelles (38 unigenes) such as the nucleolus (two unigenes), organelle envelope (six unigenes), chloroplast thylakoid membrane (two unigenes), cell wall (three unigenes), and Golgi membrane (one unigenes), cytosol (10 genes) and apoplast (five genes) influenced the fast growth of the culm (Additional file 12: Table S7).
The primers of the ten unigenes for Reverse-Transcribed PCR (RT-PCR) validation.
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