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It is presently not technically feasible to FACS-purify human β and α cells for long-term in vitro experiments due to the high-background fluorescence of human β cells caused by marked lipofuscin accumulation (Cnop et al., 2010) and the putative impact of antibody-mediated techniques on the long-term survival and function of the sorted cells.
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It is important to note that, for a given pair of recording sites, both instantaneous causality and the two directional influences may change as functions of the sorted phase-lag.
This was important to avoid the potentially toxic influence of apoptotic dyes on the function of sorted cells.
After sorting, we immediately reanalyzed samples of the sorted cells.
To examine the function of the depleted cells in anti-CCR2 and radiation treatment, we assayed sorted CCR2+Ly6chi cells from untreated or irradiated tumors for their T cell suppression function, with sorted CCR2−Ly6Cint as control (Fig. 4c).
In output the array index contains the indices of the sorted array.
The present study showed that 99.4% of the sorted cells displayed green fluorescence.
The suffix array consists of the sorted suffixes of a string.
The suffixes of the sorted elements form a sequence.
The x-axis represents the order of the sorted documents.
The gene expression profiles of the sorted B cell populations were then compared.
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