Importantly, in the last three cases (2 4), the protein sequence, structure, and function of C-terminus of Meis, which harbors the capacities for transcriptional activation and Hox interaction (Yang et al. 2000; Huang et al. 2005; Williams et al. 2005; Hyman-Walsh et al. 2010), are likely to be highly modified relative to the ancestral protein.
To unravel the actual function of the C-terminus region, we carried out functional analysis of WA PKS mutants by C-terminus deletion and site-directed mutagenesis.
To avoid interfering the function of Dvl C-terminus, we placed the myc-tag at the N-terminus.
Both herpes virus replication and V(D J recombination (but not transib element transposition) occur synchronously during the G0/G1 phase of the cell cycle coordinately with V(D J recombination, and co-coordinating interactions with cell cycle regulatory proteins [32] might also be a function of the amino terminus of herpes DBP shared with amino terminal regions of RAG-1.
Mutations at conserved sites between LATS1/2 such as LATS1-F1015/LATS2-F978, R1020/R983, F1039/F1001, and D1086/D1048 may affect function of the C-terminus.
The normal function of the N-terminus code may be to help "hide" the embryo from the mother's immune system, the team suggests in the 12 July issue of Cell.
These findings combined support an important function of the N-terminus of APP in the mammalian system as well as in C. elegans.
Additional evidence for an inhibitory function of the amino terminus can be inferred from the ability of the cortactin carboxyl terminal domain to promote N-WASP-dependent cell motility as effectively as wild type cortactin [16].
The function of the N-terminus of SpvB remains unknown but shares sequence similarity to the N-terminal domain of P. luminescens toxin TcaC, the function of TcaC is largely unclear but is thought to act as a potentiator modifying other toxin components and it is required to reproduce full toxicity of the Toxin complexes via recombinant expression [27], [28].
Furthermore, the exact function of the C-terminus remains elusive.
