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Subsequently, cumulative PDs were calculated as a function of passage number and split ratio [ 47].
Cumulative PDL was calculated as a function of passage number and split ratio (4).
Nearly 5,500 genes had expression differences as a function of passage alone.
Some genes (OTX2, RAX, CDKN2B, COL1A1, and COL1A2) even have apparent cyclical expression profiles as a function of passage.
Fig. 9 shows the expression data for a number of AMD-risk genes with two-fold or greater differences as a function of passage.
TGFBR1, whose expression increases post-confluence as a function of passage, is a target of A-83-01 A-83-01 A-83-01nsducer of TGFB1 and TGFB2 activity.
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The reduced effect of SOX9 expression in NFs is not solely a function of the passage number, since NFSCs expressing SOX9 shRNAs for several passages showed only a 10 20% reduction in growth rate.
That he is able to do so, he adds with the awareness of one accustomed to criticism, is more a function of the passage of time than in any transformation he is effecting in the fortunes of Derby County.
Interestingly, we observed that the mean DNA methylation β-value of the 100 CIMP-specific loci increased as a function of cell passage (Figure 4A and 4B).
Intriguingly, we observed that the overall DNA methylation level of the CIMP-specific loci in our stably transfected cells increases as a function of cell passage.
Decrease of telomeric DNA in human fibroblasts as a function of serial passage during in vitro aging was first demonstrated by measurement of terminal restriction fragment (TRF) length [ 10].
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