Alternatively 2), Wallace and De Yoreo employed parallel replica simulations for an extensive sampling of configurations, thus mimicking infinite relaxation times.[ 21] Between these extremes, we suggest 3) a simulated-annealing-type procedure to allow aggregate relaxation to a degree that both energy profiles and aggregate structures evolve continuously as functions of nucleus size.[ 16].
Further, the ability of a crowded environment to replace ions for conserving the structure and functions of nuclei is quite typical of a growing number of observations on macromolecular assemblies, although the nucleus is probably the largest assembly for which this phenomenon has been observed.
It is tacitly believed that the stability and functioning of nuclei in these media reflects the fact that they reproduce the ionic environment in the cytoplasm which surrounds the nucleus in vivo, but this idea is not consistent with all the available experimental evidence.
We studied the possible role in maintaining the structure and functions of nuclei in vivo of a further but frequently overlooked property of the cytoplasm, the crowding or osmotic effects caused by diffusible macromolecules whose concentration, measured in several studies, is in the range of 130 mg/ml.
It is 'internal' in orientation rather than 'external' (see the inventory of relations in Figure 9 above); that is, it serves to link a satellite text segment to the nucleus that is likely to increase the success of the speech function of the nucleus — in this case, the likelihood that the reader will believe that the printer is really bad.
Under the assumption that the length scale of the microstructure is considerably larger than the size of the nucleus, their elastic interaction energy can be expressed as a linear function of the nucleus's volume, and combined directly with the chemical nucleation driving force in the classical nucleation theory.
The primary function of the nucleus is the expression of selected subsets of the genetic information encoded in the DNA double helix.
The PAG modulates the function of the nucleus ambiguus [14].
In paper I of this series, the coherency strain energy associated with nucleus formation in an elastically anisotropic crystal of an arbitrary pre-existing microstructure was presented in a close form as a linear function of the nucleus volume, which can be incorporated directly into the classical nucleation theory.
