The affective modulation of flanker interference not only depended on but even reversed as a function of flanker-target interval.
Namely, the function prediction, based on function of flanking genes, might be very risky in Streptomyces, as Streptomyces have a relatively complex genomic organization.
This microsatellite may affect the functions of flanking regulatory elements.
It has to be noted that functional classification is not an absolute decision-making procedure regarding the likely target genes of CNSs but rather indeed an exploratory method to identify the possible likely functions of flanking genes.
M64I-associated sex reversal is instead caused by the impaired function of a flanking non-classical nuclear localization signal (NLS).
As previously described, three other putative genes have been identified adjacent to the MAT1-2: (i) a β-glucosidase homolog gene (3′ flank of MAT1-2), (ii) a homolog of a yeast gene (ORF1) with unknown function (5′ flank of MAT1-2) and (iii) a GTPase-activating homolog gene (5′ flank of MAT1-2) (Fig. 7a).
Not surprisingly, conventional QTL mapping alone, being a function of only the flanking markers, failed to accurately predict the precise regulating gene, but the QTL interval was typically identified with reasonable success in our simulation.
The differential effect of NELF-E depletion on expression of the different slp1-lacZ reporters further indicates that the relative requirement for NELF is a function of the extent of flanking cis-regulatory information.
Furthermore, the sequences of the inverted repeats thought be to essential for the function of this region that flank the minimal element are completely complementary in mouse, rat and human, [15].
A further hint towards a family-specific function of the N-terminal flanking region is that, according to our analysis, only 24% of the proteins with a PEXEL motif actually possess a standard signal sequence.
