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The biological function of duplicate genes can be happenstance due to neutral stochastic forces, for increasing transcripts for high gene expression levels (e.g., rRNA), differentiation of function (e.g., globin genes) or to protect critical genes from mutational loss.
First, the ancestral function of duplicate genes may not change with relocalization but instead could allow that function to be carried out in different subcellular compartments.
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Whether this leads to retention of function of duplicated genes or to functional diversification either at the level of gene expression or protein function remains to be determined.
It would be interesting to further investigate the function of duplicated genes with seemingly identical expression patterns.
Data on phylogeny, expression and intra-specific polymorphic information are necessary to drive hypotheses regarding the gain, loss and change of function of duplicated genes.
Our results suggest that protein subcellular relocalization is an important mechanism for the retention and gain of function of duplicated genes in animal genome evolution.
The most obvious contribution of gene duplication to organisms is that it provides genetic material to generate neo-function or sub-function while maintaining the original function of duplicated genes [ 9, 13].
Ideally, all studies concerning the function of duplicates should be integrated into evolutionary-based approaches where the history of a gene and the corresponding function have to be sought.
Substitutions can change the function of duplicated genes, and may be due to either a relaxation of purifying selection or to the action of positive selection [ 19, 23, 32, 33].
Substitutions can change the function of duplicated genes, and may be due to either a relaxation of purifying selection or the action of positive selection [ 56, 58, 59, 103].
Genome-scale analyses suggest that marked differences exist regarding the functions of duplicate genes, depending on whether they originated from small-scale or large-scale duplications [ 18].
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