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In 2009, Fuchs et al. analysed the dynamic processes involved in the differentiation and functionality of both cell types in the coculture system as a function of cultivation time [ 32].
Because weed control is the most important function of cultivation, this work should be performed at the most favourable time for weed killing, when the weeds are breaking through the soil surface.
The DNA content of monolayer cultures as a function of cultivation time was analyzed.
Since it's expression decreases as a function of cultivation time, the data indicate that ESCM must either be actively withdrawing from the cell cycle or undergoing a checkpoint delay in G1.
The goal of our optimization problem is to infer the activity of TFs as a function of cultivation parameters, such that we can optimally explain the regression coefficients, which were distilled from the observed gene expression data.
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All the parameters calculated according to Eqs. 1– 7, are expressed as time-averaged functions of cultivation time (i.e. the value at the time point of interest corrected by amount present at time zero and divided by the time from inoculation).
It was found that biomass formation and PHB accumulation were a function of various cultivation parameters.
Given that changes in gene expression levels as observed across different environmental conditions can be attributed to changes in the activity of TFs, we aim to infer the activity of TFs as a function of the cultivation parameters.
The hypo-phosphorylated or active (inhibitory) form of Rb markedly increased as a function of in vitro cultivation time.
Although primary hepatocytes initially do contain metabolic enzymes at their physiological levels immediately after isolation, most liver-specific gene expression and CYP-related functions decrease during the initial stages of cultivation.
The Fv/Fm of untreated cells as a function of the time of dark cultivation is also shown.
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