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Such genetically encoded click chemistry tools facilitate high resolution studies of protein function in live cells, viral imaging, as well as the improved design of antibody-drug conjugates.
Protein labeling with a synthetic probe is a powerful method not only for elucidating protein function in live cells but also for providing new tools to explore complicated biological processes that are associated with medical diagnosis.
The Hopkins team is adding to a growing biochemical toolkit to study native molecular function in live cells, essential equipment for a new era of biological discovery.
Therefore, we took a direct approach to measure SERCA function in live beta-cells (Duman et al. 2006; Albrecht et al. 2002).
Furthermore, local interference with myosin Va function in live wildtype mouse muscles led to fragmentation and size reduction of NMJs, exclusion of rapsyn-GFP from NMJs, reduced persistence of acetylcholine receptors in NMJs and an increased amount of punctate structures bearing internalised NMJ proteins.
The authors went on to analyze this human mutation in a zebrafish model, and assessed its function in live hearts.
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Cellular 'timers' provide an important function in living cells [1].
A We focus on the development of chemical approaches to probing molecular function in living systems.
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.
Macromolecular crowding effects may be a tool for the manipulation of protein-folding and function in living cells.
And no one has been able to get unnatural DNA to function in living cells, although progress has been made in test tubes.
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