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To calculate the probability density function, hits were summed up for ten score unit windows.
We analyzed a total of 332 enriched genes, resulting in 381 function hits.
To facilitate critical assessments, the percentage of proteins that are in concordance with the major assigned function (hits), the discordant proteins (misses) and un-known are also indicated.
The most common GO term categories in the depleted gene list (223 genes analyzed with 242 function hits) were "binding" (28%%) and "catalytic" (28%%), similar to the enriched genes (Fig. 3a).
If you were to graph a function, the roots would be the points at which the function hits the x-axis.
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The Bortezomib screen uncovered less loss-of-function hits, consistent with its superior selectivity relative to MG-132 that hits many targets in addition to the 20S proteasome.
In this case, a significant improvement in both primary (mean headache days) and secondary end points (mean drugs consumption and mean days with consumption) at 12 weeks, as well as in pain intensity and headache impact on functioning (HIT-6 and MIDAS) across all time points (4 and 12 weeks) was found.
Nonetheless, running DE on this larger starting population did not make a significant difference in the final value of the objective function (109 hits for N=27 versus 107 hits for N=19), see Figure 5.
The CAC function for hits is steeper than for correct rejections, in line with standard unequal variance signal detection models.
The 'Molecular Function' significant hits included 'myosin binding' (P=3.15E 6), 'troponin T binding' (P=2.79E 6) and 'structural constituent of muscle' (P=3.75E 4).
For BlatsP and tBlastn searches the nr dataset was utilised (which encompasses the following public databases: All non-redundant GenBank, RefSeq proteins, PDB, SwissProt, PIR, PRF) and all sequences assigned a putative function generated hits with an e-value of 1e-02 or lower.
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