Flux is the natural quantity to consider when studying metabolic pathway function and flux studies have been employed both theoretically [22,23] and experimentally [24,25].
As a function of flux threading through the ring, standard Josephson coupling yields a Φ0= h/(2e) periodic features corresponding to 2π phase difference periodicity.
In case of random media sampling, inconsistency values I have been normalized by the objective function's flux in order to make them comparable.
See also Figure 1 figure supplement 1. DOI: http://dx.doi.org/10.7554/eLife.03641.003 10.7554/eLiFigure41 figuregure 1—figure supplement 1. Biomass production as a function of flux upper bound.
However, in spite of the inadequacy of AKI as a substitute for AKII in terms of regulatory function and flux contribution, it was much more effective in emulating the former function than the latter.
It calls the tools "Fetch Reaction IDs", "Fetch objective function", ""Fetch flux constraints" and "PalssonSBML Reader" which parse the reaction identifiers, objective function, reactions constraints and reactions respectively from input SBML file.
To understand why the PSEUDO objective function improves flux predictions for the TCA cycle, we chose to investigate central carbon metabolism in greater detail using the zwf mutant as a case study.
Myocardial function, substrate flux via AR and SDH, and tissue lactate:pyruvate (L/P) ratio (a measure of cytosolic NADH/NAD+), and lactate dehydrogenase (LDH) release (a marker of IR injury) were measured.
The effects of temperature, feed concentration, and zeolite loading were investigated as functions of flux and separation factor.
