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Subsequently, the 3′-terminal of hairpin probe can function as a primer to initiate the SDA reaction, producing a large amount of triggers.
We suggest that this HB-PhaC (Scheme 4) may function as a primer of the polymerization process.
Cleavage occurs at the 5'-side of the RNA base leaving a DNA oligonucleotide with a 3'-hydroxyl that is competent to function as a primer.
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The probes structure, starting from the surface, contains a uracil cleavage region, a T7 amplification handle, a partial sequencing handle, a cluster specific sequence (spatial barcode), a semi-randomized unique molecular identifier (UMI) and an oligo-dT mRNA capture sequence which functions as a primer for cDNA synthesis.
Like the DSBR model, recombination by SDSA is initiated at DSBs, and the 3′-end of the invading strand functions as a primer for DNA synthesis (Fig. 1).
Even though it's one of the least song-centric tracks on the album, it functions as a primer on restraint that frames the listening experience.
In addition, the 3' end of ccdB functions as a primer binding site common to all vectors.
It is not known whether this oligosaccharide functions as a primer for xylan biosynthesis or as a terminator sequence [ 15, 16].
In this assay, the presence of target mir-196a2T circularizes the padlock probe and the mir-196a2T functionednctioned as a primer to initiate the next round of RCA reaction in the presence of Vent (exo-) polymerase.
It also functions as a welcome primer on the slow-food movement, which is the culinary world's version of filmmaking's back-to-basics Dogma 95 manifesto.
By far the best New York installation is at the Gagosian on West 21st Street in Chelsea, where a mere 14 paintings ring one enormous space and function as a kind of Hirst primer.
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