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The keratinocyte growth medium is serum-free and fully supplemented with growth factors and antibiotics and was designed to promote attachment, spreading and proliferation of human keratinocytes on tissue culture plates.
MDA-MB-231, T47, and HCC1937 breast cancer cell lines were cultured in Dulbecco's modified medium fully supplemented with 1% antimycotic/antibiotic (Cellgro, Mannasas, VA), and 10% Fetal Bovine Serum (Atlanta Biologicals, Lawrenceville, VA).
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Fully supplemented RWMF with no wall tendons M C P = 0, M D P ≠ 0 and M B P ≠ 0.
CCL-171 cells were first placed under replicative quiescence in DMEM media with 0.1% serum for 48 h and then exposed to fully supplemented media, DMEM with 10% serum, and incubated with BMP4 for 1, 3, 6, 12, 24 or 48 h.
Bone marrow-derived macrophages (BMDM) were differentiated from freshly isolated bone marrow cells in fully supplemented RPMI-1640 together with 10% L929-conditioned medium.
Yeast strains were cultured in YPD medium or SD-based media supplemented with appropriate amino acids; fully supplemented medium containing all amino acids plus uracil and adenine is referred to as SC.
However, the treatment of the HUVEC pools with 100nM recombinant BDNF at 24 hours post-transfection (at the same point as treatment with either fully supplemented media or SFD conditions), did not induce significant rescue from SFD-induced cell death, as measured by both the quantification of active caspase-3 and 7 and the ADP ATP ratio (data not shown).
In RT-PCR and clonogenic survival experiments, chetomin was added in a concentration of 150 nM to fully supplemented medium four hours before treatment with hypoxia.
B cells were incubated with nanoparticles (50 µg/mL) in fully supplemented RPMI 1640 (ATCC; Manassas, VA) for 2 h.
Cells were then left for a further 24hrs before treatment with either survival factor deprived conditions of basal media without supplements (EBM-2) or fully supplemented media without antibiotics (EGM-2) for 24 hrs.
DNA replication in Plx1-depleted extracts could be fully restored when supplemented with recombinant Plx1.
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