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Although of tremendous value, intestinal organoid culture systems have not yet fully recapitulated the anatomy or physiology of the in vivo intestinal epithelium.
Therefore, the KOSR effect can be fully recapitulated with lipid-rich BSA (AlbuMAX).
As shown in Figure 4D, treatment with PMA alone fully recapitulated the role of anti-CD3.
In addition, it remains possible that there are determinants of mRNA expression, stability and translation within the gene coding sequences that may not be fully recapitulated using the reporter design in this study.
Moreover, loss of FOXM1 also leads to centrosome amplification and mitotic catastrophe, a phenotype fully recapitulated by MEIS2 depletion.
Furthermore, in many cases the complexity of ligand receptor binding interactions cannot be fully recapitulated with short peptides.
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Furthermore, the exogenous addition of soluble factors was not able to fully recapitulate this phenotype that was imparted by shear stress exposure.
Although murine models do not fully recapitulate human infection, they are commonly used to evaluate EHEC vaccines and the immune/protective responses elicited in the host.
However, in transgenic assays, the GCR was unable to fully recapitulate all aspects of the endogenous Hoxd expression patterns during distal limb development.
Because this expression profile did not fully recapitulate the previously reported activation stages of monocytes, we proposed that the newly generated monocytes represented a unique and distinct state of activation.
They were therefore unlikely to fully recapitulate the biology of primary in vivo tumor development.
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